New Generation Sequencing (NGS) technologies and qRT-PCR associated with specific bioinformatics programs aim to discover and validate new DNA/RNA/genomics/transcriptomics biomarkers. The objective: developing new diagnostics in veterinary applications.


Gene expression profiles (transcriptomes) through sequencing technologies (New Generation Sequencing, NGS) and specific bioinformatics tools aim to the acquisition of new DNA/RNA/genomics/transcriptomics data. This quantitative process helps for identifying all the biomarkers involved in each physiopathological situation in an exhaustive and sensitive manner. However, the genes are either already known or not, the genome of the organism completely sequenced or not..


But, specific bioinformatics programs and database are fully required for analyzing the results generated by these New Generation Sequencing (NGS) systems, including sequence analysis, gene annotation, computational evolutionary biology, measuring biodiversity, analysis of regulation, analysis of protein expression, analysis of mutation, etc.


After these bioinformatics treatments, all the results can be implemented in an interactive database and can be compared with other gene expression profiles (transcriptomes) established through sequencing. Thus, each user can easily search and compare gene expression profiles (transcriptomes), and can select sets of specific biomarkers dedicated to a tissue or a pathology...


To validate these biomarkers or in a high throughput routine, quantitative Real-Time PCR can be used for monitoring global patterns of gene expression. This technique provides an absolute quantitative analysis of gene expression and a rapid and precise confirmation of genetic changes.


Quantitative Real-Time PCR:


  Opens internal link in current windowIs the most sensitive technique for mRNA detection and quantification,
  Opens internal link in current windowCan be used to quantify mRNA levels from much smaller samples,
  Opens internal link in current windowGives more accurate measurements and is much better adapted to analyses performed on
      large numbers of samples,
  Opens internal link in current windowHas been successfully developed and has become a reliable and easy to use standard method
      for the detection and/or quantification of nucleic acid sequences,
  Opens internal link in current windowReduces hands-on time and increases reliability,
  Opens internal link in current windowHas been adopted for a wide range of new applications.


If you require more information, please contact the DIAG4ZOO Team (Tel: +33 (0)467 419 748, Email:




Biomarkers and diagnostics leading to animal healthcare management within the challenge of breeding in Quality, Safety, Secure conditions, and Traceability.